We strive to improve glycoprotein therapeutics by custom-designed glycans
Glycans on the therapeutic glycoproteins are known to affect the product's stability, PK/PD, and activity. For example, N-glycans on human IgG have important roles in modulating its secretion as well as the effector functions through antibody-dependent cellular cytotoxicity (ADCC), and this discovery has led to the development of several new cancer therapeutic antibodies with improved efficacy.
With the emergence of the precise gene editing technologies it is now possible to perform comprehensive glycoengineering on glycoprotein therapeutics produced in mammalian cells, and GlycoDisplay has developed extensive design strategies for novel glycans on therapeutic glycoproteins. With GlycoDisplay's glycoengineering technology we can design and express optimal glycan structures on preferred proteins, offering our customers to effectively develop novel glycoengineered biologics.
Examples of glycoengineered biologics GlycoDisplay has worked include EPO with homogeneous glycans, IgG with homogeneous glycans, and lysosomal replacement enzymes with extended half-life.
Glycoengineering of cell lines
We utilize precise gene engineering techniques to perform individual or combined gene knock out (KO) and knock in (KI) in order to express desired glycan
structures in the cell lines of interest.
Our proprietary design matrices covers N-linked, O-linked, and glycosaminoglycans.
To begin, we will discuss with customers on customers' specific needs including the design of glycans and gene editing strategies. Once a service agreement has been established, we will proceed to design and validate gRNA (if it is not already available). We will then use the validated gRNA for further gene editing, production of single cell clones, and validation. The timeline for obtaining single-cell clones varies depending on the specific design of the gene engineering and cell line. For more information, please don't hesitate to contact us.
Recombinant protein expression on glycoengineered cells
Purification/characterisation of glycoproteins
Our services assist customers in expressing recombinant proteins using available cell libraries in HEK 293 cell lines, as well as custom-
glycoengineered cell lines. The resulting glycoproteins can be purified and characterized using various methods, such as SDS-PAGE, Western Blot, and activity assays. Additionally, glycan profiling can be performed using techniques such as MS, MALDI-TOF, and lectin staining.