Jaroentomeechai T, Karlsson R, Goerdeler F, Teoh FKY, Grønset MN, de Wit D, Chen YH, Furukawa S, Psomiadou V, Hurtado-Guerrero R, Vidal-Calvo EE, Salanti A, Boltje TJ, van den Bos LJ, Wunder C, Johannes L, Schjoldager KT, Joshi HJ, Miller RL, Clausen H, Vakhrushev SY, Narimatsu Y. (2024) Mammalian cell-based production of glycans, glycopeptides and glycomodules. Nat Commun 15(1):9668- doi: 10.1038/s41467-024-53738-9.
Vidal-Calvo EE, Martin-Salazar A, Choudhary S, Dagil R, Raghavan SSR, Duvnjak L, Nordmaj MA, Clausen TM, Skafte A, Oberkofler J, Wang K, Agerbæk MØ, Løppke C, Jørgensen AM, Ropac D, Mujollari J, Willis S, Garcias López A, Miller RL, Karlsson RTG, Goerdeler F, Chen Y-H, Colaço AR, Wang Y, Lavstsen T, Martowicz A, Nelepcu I, Marzban M, Oo HZ, Ørum-Madsen MS, Wang Y, Nielsen MA, Clausen H, Wierer M, Wolf D, Gögenur I, Theander TG, Al-Nakouzi N, Gustavsson T, Daugaard M, Salanti A (2024). Tumor-agnostic cancer therapy using antibodies targeting oncofetal chondroitin sulfate. Nat Commun. 15(1):7553. doi: 10.1038/s41467-024-51781-0.
Elzinga J, Narimatsu Y, de Haan N, Clausen H, de Vos WM, Tytgat HLP (2024): Binding of Akkermansia muciniphila to mucin is O-glycan specific. Nat Commun.15(1):4582. doi: 10.1038/s41467-024-48770-8.
Sørensen DM, Büll C, Madsen TD, Lira-Navarrete E, Clausen TM, Clark AE, Garretson AF, Karlsson R, Pijnenborg JFA, Yin X, Miller RL, Chanda SK, Boltje TJ, Schjoldager KT, Vakhrushev SY, Halim A, Esko JD, Carlin AF, Hurtado-Guerrero R, Weigert R, Clausen H, Narimatsu Y (2023): Identification of global inhibitors of cellular glycosylation. Nat Commun. 20;14(1):948. doi: 10.1038/s41467-023-36598-7
Anggara K, Sršan L, Jaroentomeechai T, Wu X, Rauschenbach S, Narimatsu Y, Clausen H, Ziegler T, Miller RL, Kern K. (2023) Direct observation of glycans bonded to proteins and lipids at the single-molecule level. Science. 382(6667):219-223. doi: 10.1126/science.adh3856.
Chen Y-H, Tian W, Yasuda M, Ye Z, Song M, Mandel U, Kristensen C, Povolo L, Marques A R, Caval T, Heck A J R, Sampaio J L, Johannes L, Tsukimura T, Desnick R, Vakhrushev S Y, Yang Z, Clausen H (2023): A universal GlycoDesign for lysosomal replacement enzymes to improve circulation time and biodistribution. Front. Bioeng. Biotechnool, 11:1128371. doi: 10.3389/fbioe.2023.1128371
Nason R, Bull C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, Sørensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, Haan C, Vincentelli R, Henrissat B, Vakhrushev S, Clausen H, Narimatsu Y (2021): Display of the human mucinome with defined O-glycans by gene engineered cells. Nat Commun 1;12(1):4070. DOI: 10.1038/s41467-021-24366-4.
Narimatsu Y, Bull C, Chen Y-H, Wandall HH, Yang Z, Clausen H (2021): Genetic glycoengineering in mammalian cells. J Biol Chem, 19;296:100448. DOI: 10.1016/j.jbc.2021.100448.
Schjoldager KT, Narimatsu Y, Joshi HJ, Clausen H (2020): Global view of human protein glycosylation pathways and functions. Nat Rev Mol Cell Biol, 21(12):729-749. DOI:10.1038/s41580-020-00294-x
Narimatsu Y, Joshi H, Nason R, Van Coillie J, Karlsson R, Sun L, Ye Z, Chen Y-H, Schjoldager KT, Steentoft C, Furukawa S, Bensing BA, Sullam PM, Thompson AJ, Paulson JC, Bull C, Adema GJ, Mandel U, Hansen L, Bennett EP, Varki A, Vakhrushev SY, Yang Z, Clausen H (2019): An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells. Mol Cell 75:1-14. DOI: 10.1016/j.molcel.2019.05.017
Tian W, Ye Z, Wang S, Schulz MA, Van Coillie J, Sun L, Chen Y-H, Narimatsu Y, Hansen L, Kristensen C, Mandel U, Bennett EP, Jabbarzadeh-Tabrizi S, Schiffmann R, Shen J-S, Vakhrushev SY, Clausen H, Yang Z. (2019): The Glycosylation Design Space for Recombinant Lysosomal Replacement Enzymes Produced in CHO Cells. Nat Commun. 10:1785. DOI: 10.1038/s41467-019-09809-3
Chen Y-H, Narimatsu Y, Clausen TM, Gomes C, Karlsson R, Steentoft C, Spliid CB, Gustavsson T, Salanti A, Persson A, Malmström A, Willén W, Ellervik U, Bennett EP, Mao Y, Clausen H & Yang Z (2018): The GAGOme: a cell-based library of displayed glycosaminoglycans. Nat Methods 15(11):881-888. DOI: 10.1038/s41592-018-0086-z
Schulz MA, Tian W, Mao Y, van Coillie J, Sun L, Larsen JS, Chen YH, Kristensen C, Vakhrushev SY, Clausen H, Yang Z. (2018): Glycoengineering design options for IgG1 in CHO cells using precise gene editing. Glycobiology 1;28(7):542-549. DOI: 10.1093/glycob/cwy022
Narimatsu Y, Joshi HJ, Zhang Y, Gomes C, Chen Y-H, Lorenzetti F, Furukawa S, Schjoldager K, Hansen L, Clausen H, Bennett EP, Wandall HH (2018): A validated gRNA library for CRISPR/Cas9 targeting of the human glycosyltransferase genome. Glycobiology 1;28(5):295-305. DOI: 10.1093/glycob/cwx101
Lonowski LA, Narimatsu Y, Riaz A, Delay CE, Yang Z, Niola F, Duda K, Ober EA, Clausen H, Wandall HH, Hansen SH, Bennett EP, Frödin M (2017): Genome editing using FACS enrichment of nuclease-expressing cells and idel detection by amplicon analysis. Nat Protocols 12(3):581-603. DOI: 10.1038/nprot.2016.165
Yang Z, Wang S, Halim A, Schulz MA, Frodin M, Rahman SH, Vester-Christensen MB, Behrens C, Kristensen C, Vakhrushev SY, Bennett EP, Wandall HH, Clausen H. (2015): Engineered CHO cells for production of diverse, homogeneous glycoproteins. Nat Biotechnol 33:842-4. DOI: 10.1038/nbt.3280
