Using targeted or random approaches a plurality of glycovariants of a biologic candidate may be generated for screening. The protein of interest will be expressed transiently in glycoengineered HEK293 cell lines under serum-free suspension culture conditions. Expressed glyco-variant proteins may be purified or used directly for assaying. Screening for optimized drug features will allow the selection of lead candidates for clinical development.
When an optimal drug candidate has been identified, the design matrix of the underlying glyco-engineered cell line may be applied to develop a high-yielding stable production cell line.
The HEK293 system is our preferred host system for glycovariant screening, but the versatility of our technology allows engineering of any glycoform into any cell line, including the favorite cell line of our partners.